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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: TOMM40 Genetic Variants Cause Neuroinflammation in Alzheimer’s Disease
doi: 10.3390/ijms24044085
Figure Lengend Snippet: TOMM40 genetic variants induce the secretion of pro-inflammatory cytokines in microglial cells, leading to cell death of hippocampal neurons. ( A ) Compared to control cells or cells expressing WT TOMM40, BV2 microglial cells’ expression of (F113L) or (F131L) TOMM40 significantly increased secretion of pro-inflammatory IL-1β, IL-6, or TNF-α in culture medium of BV2 microglial cells. ( B ) Culture medium (CM) of HT22 hippocampal neurons was replaced with CM from BV2 microglial cells transfected with cDNA of WT, (F113L) or (F131L) TOMM40. One day after replacement, CM of BV2 microglia cells expressing mutant (F113L) or (F131L) TOMM40 significantly reduced cell viability of HT22 hippocampal neurons. Each bar represents mean ± S.D. of four experiments. Each experiment was performed in triplicate. * p < 0.05 or ** p < 0.01 compared to control BV2 microglial cells or HT22 hippocampal neurons.
Article Snippet: BV2 mouse microglial cells and
Techniques: Control, Expressing, Transfection, Mutagenesis